Simulation of STED microscopy
This application note demonstrates how our modeling software SimphoSOFT® allows a user to model and optimize the performance of STED using the SimphoSOFT features of multiple beams and stimulated emission. SimphoSOFT can be used
- to help design STED microscopes, and
- to optimize the performance of fluorescent probes used in STED microscopy.
FWHM of the fluorescing spot will be greatly reduced at a time of 60 ps (after the Donut STED pulse has passed through the sample) compared to a time of 5 ps (which is just before the Donut pulse). More analysis and details are in the application note.
SimphoSOFT® can be purchased as a single program and can be also configured with Energy Transfer add-on , Multi-Beam add-on , Optimization add-on , Z-scan add-on , and MPA Info+ add-on for an additional charge. Please, contact our sales staff for more information
Simphotek offers a full free trial version for 30-day evaluation.
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