diffraction

The original naturally-occurring green fluorescent protein (GFP) is a protein found in a type of jellyfish, Aequorea victoria. Green fluorescent protein from this jellyfish exhibits fluorescence emission in the green region of the optical spectrum at about 509 nm after excitation with blue light at about 395 nm. A smaller excitation absorption peak is also present at 475 nm for the natural GFP. Genetically modified GFPs have been produced that are more stable and that have the excitation absorption peak shifted to about 488 nm. The gene for modified GFP can be grafted to other target protein genes and expressed in living cells without significantly affecting cell functions. The modified cells produce the target protein with GFP attached. The GFP can thereby act as an optical fluorescent marker for the presence of the target protein.







SimphoSOFT logo SimphoSOFT® supports modeling laser beam interaction with green fluorescent protein.


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